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Image Search Results
Journal: Biology
Article Title: Konjac Ceramide (kCer)-Mediated Signal Transduction of the Sema3A Pathway Promotes HaCaT Keratinocyte Differentiation
doi: 10.3390/biology11010121
Figure Lengend Snippet: kCer-induced HaCaT cell differentiation and migration. Short-term cell culture was used for the HaCaT cell migration assay. Cell migration activity was determined quantitatively using ImageJ after GIEMSA’S AZUR EOSIN Methylene Blue-staining of the transwell filter membrane (left images in ( A , B )). Cells that migrated to the opposite side of the transwell membrane were immunostained with anti-involucrin antibody (right image in ( A , B )). ( C ) The differentiation effect of Sema3A (100 nM) and kCer (25 μM) on HaCaT cells after short-term culture was compared in the presence or absence of 10 μM histamine and quantified using Image J. Data are presented as means ± SD ( n = 3, ** p < 0.01). Scale bar = 100 μm.
Article Snippet: A cell migration assay was performed using a
Techniques: Cell Differentiation, Migration, Cell Culture, Cell Migration Assay, Activity Assay, Staining, Membrane